Mol Plant , IF:12.084 , 2021 May doi: 10.1016/j.molp.2021.05.005
The miR166- SlHB15A Regulatory Module controls Ovule Development and Parthenocarpic Fruit Set under Adverse Temperatures in Tomato.
Institute of Plant Sciences Paris-Saclay, INRAE, CNRS, Universite Paris-Saclay, Orsay 91405, France.; Institut Jean-Pierre Bourgin, INRAE, Versailles, France.; HM Clause, Mas Saint-Pierre, quartier La Galine, Saint-Remy de Provence 13210, France.; Hazera Seeds Ltd, Berurim M.P. Shikmim 7983700, Israel.; Institute of Plant Sciences Paris-Saclay, INRAE, CNRS, Universite Paris-Saclay, Orsay 91405, France. Electronic address: abdelhafid.bendahmane@inrae.fr.
Fruit set is inhibited by adverse temperatures, with consequences on yield. We isolated a tomato mutant producing fruits under non-permissive hot temperatures and identified the causal gene as SlHB15A, belonging to class-III homeodomain leucine-zipper transcription factors (HD-ZipIII). SlHB15A loss-of-function mutants display aberrant ovule development that mimics transcriptional changes occurring in fertilized ovules and leads to parthenocarpic fruit set under optimal and non-permissive temperatures, in field and glasshouse conditions. Under cold growing condition, SlHB15A is subjected to conditional haploinsufficiency and recessive dosage sensitivity controlled by microRNA 166 (miR166). Knockdown of SlHB15A alleles by miR166 leads to a continuum of aberrant ovules correlating with parthenocarpic fruit set. Consistent with this, plants harboring SlHB15A-miRNA166 resistant allele developed normal ovules and were unable to set parthenocarpic fruit under cold condition. DNA affinity purification sequencing (DAP-seq) and RNAseq analyses revealed SlHB15A is a bifunctional transcription factor, expressing in the ovule integument. SlHB15A binds to the promoters of auxin genes to repress auxin signaling and to ethylene genes to activate their expression. Survey of tomato genetic biodiversity identified pat and pat-1, two historical parthenocarpic mutants, as alleles of SlHB15A. Our finding demonstrates the role of SlHB15A as a sentinel to prevent fruit set in the absence of fertilization and provides a mean to enhance fruiting under extreme temperatures.
PMID: 33964458
Plant Cell , IF:9.618 , 2021 May doi: 10.1093/plcell/koab122
Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress.
Departamento de Biologia Molecular y Bioquimica. Instituto de Hortofruticultura Subtropical y Mediterranea "La Mayora", Universidad de Malaga-Consejo Superior de Investigaciones Cientificas (IHSM-UMA-CSIC), Universidad de Malaga, 29071, Malaga, Spain.; Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, 201602, Shanghai, China.; Plant Sciences, Rothamsted Research, AL5 2JQ, Harpenden, UK.; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.; VIB Center for Plant Systems Biology, 9052, Ghent, Belgium.; Departamento de Biotecnologia Microbiana y de Plantas; Centro de Investigaciones Biologicas Margarita Salas-CSIC; 28040, Madrid, Spain.; Departamento de Cristalografia y Biologia Estructural, Instituto de Quimica Fisica "Rocasolano", Consejo Superior de Investigaciones Cientificas, 28006, Madrid, Spain.; College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, China.; Institute of Science and Technology (IST), 3400, Klosterneuburg, Austria.; Department of Botany, The University of British Columbia, BC V6T 1Z4, Vancouver, Canada.
Endoplasmic reticulum-plasma membrane contact sites (ER-PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER-PM protein tether synaptotagmin1 (SYT1) exhibit decreased plasma membrane (PM) integrity under multiple abiotic stresses such as freezing, high salt, osmotic stress and mechanical damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an ER-PM tether that also functions in maintaining PM integrity. The ER-PM CS localization of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated by abiotic stress. Lipidomic analysis revealed that cold stress increased the accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to wild type while the levels of most glycerolipid species remain unchanged. Additionally, the SYT1-green fluorescent protein (GFP) fusion preferentially binds diacylglycerol in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol at the PM produced during episodes of abiotic stress.
PMID: 33944955
Front Microbiol , IF:4.235 , 2021 , V12 : P660134 doi: 10.3389/fmicb.2021.660134
Leveraging Pseudomonas Stress Response Mechanisms for Industrial Applications.
AgBiome Inc., Research Triangle Park, NC, United States.; Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United States.
Members of the genus Pseudomonas are metabolically versatile and capable of adapting to a wide variety of environments. Stress physiology of Pseudomonas strains has been extensively studied because of their biotechnological potential in agriculture as well as their medical importance with regards to pathogenicity and antibiotic resistance. This versatility and scientific relevance led to a substantial amount of information regarding the stress response of a diverse set of species such as Pseudomonas chlororaphis, P. fluorescens, P. putida, P. aeruginosa, and P. syringae. In this review, environmental and industrial stressors including desiccation, heat, and cold stress, are cataloged along with their corresponding mechanisms of survival in Pseudomonas. Mechanisms of survival are grouped by the type of inducing stress with a focus on adaptations such as synthesis of protective substances, biofilm formation, entering a non-culturable state, enlisting chaperones, transcription and translation regulation, and altering membrane composition. The strategies Pseudomonas strains utilize for survival can be leveraged during the development of beneficial strains to increase viability and product efficacy.
PMID: 34040596
Physiol Plant , IF:4.148 , 2021 May doi: 10.1111/ppl.13457
Freezing stress damage and growth viability in Vaccinium macrocarpon Ait. bud structures.
Department of Horticulture, University of Wisconsin, Madison, WI, USA.
After a freezing event, it can be challenging to extrapolate levels of freezing damage to plant growth viability based on the presence or absence of symptoms in specific bud tissues. This study investigated the relationship between freezing damage in terminal buds during ecodormancy and their viability during the subsequent growing season. We identified the bud structure that best explained this relationship, and developed a model to explain the changes in bud cold hardiness. Vertical shoots (uprights) of Vaccinium macrocarpon Ait. were sampled in central Wisconsin during Spring of 2018 and 2019. Sets of uprights with terminal buds were subjected to controlled freezing tests, followed by either visual freeze damage evaluation or assessment of shoot viability by growth assays. We determined the Browning Lethal-Temperature50 (BLT50 ), as temperature for 50% damage (tissue browning) at each bud structure, and Growth Lethal-Temperature50 (GLT50 ) temperature where 50% reduction in growth viability occurred. Two models were constructed to explain: (1) bud structure damage and growth viability, and (2) GLT50 's seasonal changes, representing the cold hardiness variations, and environmental factors. The correlation between the BLT50 and GLT50 values was closest for the bud scales and bud axis, indicating the better correspondence between levels of freezing damage with the impact on the growth potential. In addition, the latter was also the most suitable candidate for modeling due to easier damage evaluation. The freezing stress damage of the bud axis explained comparatively best the resulting growth viability. Seasonal changes in GLT50 were best explained by temperature indices based on daily minimum and on maximum temperatures over 10-day periods. However, among the model components, daily maximum temperatures had the greatest influence on V. macrocarpon cold hardiness changes during ecodormancy.
PMID: 33982304
Plant Cell Physiol , IF:4.062 , 2021 May doi: 10.1093/pcp/pcab060
RsmD, a Chloroplast rRNA m2G Methyltransferase, Plays a Role in Cold Stress Tolerance via Possibly Affecting Chloroplast Translation in Arabidopsis.
Department of Applied Biology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, South Korea.; Faculty of Forestry Agriculture, Tay Nguyen University.; The Western Highlands Agriculture and Forestry Science Institute, Buon Ma Thuot City, DakLak Province 63000, Vietnam.
Ribosomal RNA (rRNA) methylation is a pivotal process in the assembly and activity of ribosomes, which in turn play vital roles in the growth, development, and stress responses of plants. Although few methyltransferases responsible for rRNA methylation have been identified in plant chloroplasts, the nature and function of these enzymes in chloroplasts remain largely unknown. In this study, we characterized Arabidopsis RsmD (At3g28460), an ortholog of the methyltransferase responsible for N2-methylguanosine (m2G) modification of 16S rRNA in Escherichia coli. Confocal microscopic analysis of an RsmD-GFP fusion protein revealed that RsmD is localized to chloroplasts. Primer extension analysis indicated that RsmD is responsible for m2G methylation at position 915 in the 16S rRNA of Arabidopsis chloroplasts. Under cold stress, rsmd mutant plants exhibited retarded growth, i.e., had shorter roots, lower fresh weight, and pale-green leaves, compared with wild-type plants. However, these phenotypes were not detected in response to drought or salt stress. Notably, the rsmd mutant was hypersensitive to erythromycin or lincomycin and accumulated fewer chloroplast proteins compared with the wild type, suggesting that RsmD influences translation in chloroplasts. Complementation lines expressing RsmD in the rsmd mutant background recovered wild-type phenotypes. Importantly, RsmD harbored RNA methyltransferase activity. Collectively, the findings of this study indicate that RsmD is a chloroplast 16S rRNA methyltransferase responsible for m2G915 modification that plays a role in the adaptation of Arabidopsis to cold stress.
PMID: 34015128
Rice (N Y) , IF:3.912 , 2021 May , V14 (1) : P42 doi: 10.1186/s12284-021-00485-w
OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45-1 at the Seedling Stage in Rice.
Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, No. 5333 Xi'an Road, Changchun, 130062, China.; Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Milyang, 50463, Republic of Korea.; Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, No. 5333 Xi'an Road, Changchun, 130062, China. jwz1975@jlu.edu.cn.; Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, No. 5333 Xi'an Road, Changchun, 130062, China. duxinglin2004@163.com.
BACKGROUND: Cold stress is the main abiotic stress in rice, which seriously affects the growth and yield of rice. Identification of cold tolerance genes is of great significance for rice to solve these problems. GATA-family transcription factors involve diverse biological functions, however, their role in cold tolerance in rice remains unclear. RESULTS: In this study, a GATA-type zinc finger transcription factor OsGATA16, which can improve cold tolerance, was isolated and characterized from rice. OsGATA16 belongs to OsGATA subfamily-II and contains 11 putative phosphorylation sites, a nuclear localization signal (NLS), and other several conserved domains. OsGATA16 was expressed in all plant tissues, with the strongest in panicles. It was induced by cold and ABA treatments, but was repressed by drought, cytokinin and JA, and acted as a transcriptional suppressor in the nucleus. Overexpression of OsGATA16 improves cold tolerance of rice at seedling stage. Under cold stress treatments, the transcription of four cold-related genes OsWRKY45-1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT). Interestingly, OsGATA16 bound to the promoter of OsWRKY45-1 and repressed its expression. In addition, haplotype analysis showed that OsGATA16 polarized between the two major rice subspecies japonica and indica, and had a non-synonymous SNP8 (336(G)) associated with cold tolerance. CONCLUSION: OsGATA16 is a GATA transcription factor, which improves cold tolerance at seedling stage in rice. It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45-1, OsSRFP1, OsCYL4 and OsMYB30. Additionally, OsGATA16 has a non-synonymous SNP8 (336(G)) associated with cold tolerance on CDS region. This study provides a theoretical basis for elucidating the mechanism of cold tolerance in rice and new germplasm resources for rice breeding.
PMID: 33982131
Plant Physiol Biochem , IF:3.72 , 2021 May , V165 : P94-103 doi: 10.1016/j.plaphy.2021.05.020
Transcriptomic responses and physiological changes to cold stress among natural populations provide insights into local adaptation of weeping forsythia.
Innovation Platform of Molecular Biology, College of Landscape and Art, Henan Agricultural University, Zhengzhou, China. Electronic address: liyongrui1@126.com.; Innovation Platform of Molecular Biology, College of Landscape and Art, Henan Agricultural University, Zhengzhou, China.; U.S. Forest Service, Rocky Mountain Research Station, 2500 S. Pine Knoll Dr., Flagstaff, AZ, USA.
Genetic mechanisms of species local adaptation are an emerging topic of great interest in evolutionary biology and molecular ecology. In this study, we compared the changes of physiological and phenotypic indexes and gene expression of four weeping forsythia populations under cold stress through a common garden experiment. Physiological and phenotypic results showed that there were differences in cold tolerance among populations. cold tolerance of high the latitude population (HBWZ) was the strongest, followed by the middle latitude population (SXWL), while the low latitude populations (SXHM) and (SXLJ) expressed the weakest cold tolerance. We identified significant differences in gene expression of cold tolerance related pathways and ontologies, including genes of oxylipin and isoquinoline alkaloid biosynthetic process, galactose, tyrosine and unsaturated fatty acids metabolism, among these populations under the same experimental temperature treatments. Even under the same degree of stress, there were notable differences in gene expression among natural populations. In this study, we present a working model of weeping forsythia populations which evolved in the context of different intensities of cold stress. Our study provides new insights for comprehending the genetic mechanisms of local adaptation for non-model species.
PMID: 34034164
Tree Physiol , IF:3.655 , 2021 May , V41 (5) : P771-790 doi: 10.1093/treephys/tpaa147
Genome-wide analysis of long noncoding RNAs affecting floral bud dormancy in pears in response to cold stress.
College of Horticulture, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Cangshan District, Fuzhou 350002, China.; Economic Crop Station, Agricultural and Rural Bureau of Yongtai County, 32 Tashan Road, Yongtai Country, Fuzhou 350700, China.; Lianjiang State-Owned Forest Farm in Fujian Province, 31 Xifeng Road, Lianjiang Country, Fuzhou 350500, China.
The versatile role of long noncoding RNAs (lncRNAs) in plant growth and development has been established, but a systematic identification and analysis of lncRNAs in the pear has not been reported. Bud dormancy is a crucial and complicated protective mechanism for plants in winter. The roles of lncRNAs in the dormancy process remain largely unclear. In this study, we induced pear floral buds to enter into different dormant statuses by simulating four different chilling accumulation conditions. Then, a time series of RNA-seq analysis was performed and we identified 7594 lncRNAs in Pyrus pyrifolia (Burm. F.) Nakai that have not been identified. The sequence and expression of the lncRNAs were confirmed by PCR analysis. In total, 6253 lncRNAs were predicted to target protein-coding genes including 692 cis-regulated pairs (596 lncRNAs) and 13,158 trans-regulated pairs (6181 lncRNAs). Gene Ontology analysis revealed that most of lncRNAs' target genes were involved in catalytic activity, metabolic processes and cellular processes. In the trend analysis, 124 long-term cold response lncRNAs and 80 short-term cold response lncRNAs were predicted. Regarding the lncRNA-miRNA regulatory networks, 59 lncRNAs were identified as potential precursors for miRNA members of 20 families, 586 lncRNAs were targets of 261 pear miRNAs and 53 lncRNAs were endogenous target mimics for 26 miRNAs. In addition, three cold response lncRNAs, two miRNAs and their target genes were selected for expression confirmed. The trend of their expression was consistent with the predicted relationships among them and suggested possible roles of lncRNAs in ABA metabolic pathway. Our findings not only suggest the potential roles of lncRNAs in regulating the dormancy of pear floral buds but also provide new insights into the lncRNA-miRNA-mRNA regulatory network in plants.
PMID: 33147633
PLoS One , IF:2.74 , 2021 , V16 (5) : Pe0249108 doi: 10.1371/journal.pone.0249108
Integrating transcriptome and metabolome analyses of the response to cold stress in pumpkin (Cucurbita maxima).
Shandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science & Technology, Qingdao, Shandong, China.; Qingdao Institute of Agricultural Science Research, Qingdao, Shandong, China.
Cucurbita maxima belong to the genus Cucurbita and are of nutritional and economic importance. Physiological activity, transcriptome, and metabolome analyses of leaf samples from the C. maxima inbreding line IL7 treated at 5 degrees C and 25 degrees C were performed. Cold stress resulted in a significant increase in the malondialdehyde content, relative electrical conductivity, soluble protein, sugar content, and catalase activity. A total of 5,553 differentially expressed genes were identified, of which 2,871 were up-regulated and 2,682 down-regulated. In addition, the transcription of differentially expressed genes in the plant hormone signal transduction pathway and transcription factor families of AP2/ERF, bHLH, WRKY, MYB, and HSF was activated. Moreover, 114 differentially expressed metabolites were identified by gas chromatography time-of-flight mass spectrometry, particularly through the analysis of carboxylic acids and derivatives, and organooxygen compounds. The demonstration of a series of potential metabolites and corresponding genes highlighted a comprehensive regulatory mechanism. These findings will provide novel insights into the molecular mechanisms associated with the response to cold stress in C. maxima.
PMID: 33956796
PLoS One , IF:2.74 , 2021 , V16 (5) : Pe0248089 doi: 10.1371/journal.pone.0248089
Genetic mapping and identification of a QTL determining tolerance to freezing stress in Fragaria vesca L.
Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, As, Norway.; Department of Biotechnology, Faculty of Applied Ecology, Agricultural Sciences & Biotechnology, Inland Norway University of Applied Sciences, Hamar, Norway.; Department of Biosciences, Section for Genetics and Evolutionary Biology, University of Oslo, Oslo, Norway.; Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, Indiana, United States of America.; Graminor Breeding Ltd., Ridabu, Norway.; Department of Plant Sciences, Norwegian University of Life Sciences, As, Norway.; Department of Genetics, Genomics and Breeding, NIAB-EMR, East Malling, Kent, United Kingdom.; Natural Resources Institute, University of Greenwich, Medway Campus, Chatham Maritime, Kent, United Kingdom.
Extreme cold and frost cause significant stress to plants which can potentially be lethal. Low temperature freezing stress can cause significant and irreversible damage to plant cells and can induce physiological and metabolic changes that impact on growth and development. Low temperatures cause physiological responses including winter dormancy and autumn cold hardening in strawberry (Fragaria) species, and some diploid F. vesca accessions have been shown to have adapted to low-temperature stresses. To study the genetics of freezing tolerance, a F. vesca mapping population of 143 seedlings segregating for differential responses to freezing stress was raised. The progeny was mapped using 'Genotyping-by-Sequencing' and a linkage map of 2,918 markers at 851 loci was resolved. The mapping population was phenotyped for freezing tolerance response under controlled and replicated laboratory conditions and subsequent quantitative trait loci analysis using interval mapping revealed a single significant quantitative trait locus on Fvb2 in the physical interval 10.6 Mb and 15.73 Mb on the F. vesca v4.0 genome sequence. This physical interval contained 896 predicted genes, several of which had putative roles associated with tolerance to abiotic stresses including freezing. Differential expression analysis of the 896 QTL-associated gene predictions in the leaves and crowns from 'Alta' and 'NCGR1363' parental genotypes revealed genotype-specific changes in transcript accumulation in response to low temperature treatment as well as expression differences between genotypes prior to treatment for many of the genes. The putative roles, and significant interparental differential expression levels of several of the genes reported here identified them as good candidates for the control of the effects of freezing tolerance at the QTL identified in this investigation and the possible role of these candidate genes in response to freezing stress is discussed.
PMID: 34019543
PeerJ , IF:2.379 , 2021 , V9 : Pe11428 doi: 10.7717/peerj.11428
Comparative transcriptome profiling of a resistant vs susceptible bread wheat (Triticum aestivum L.) cultivar in response to water deficit and cold stress.
The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation.; Novosibirsk State University, Novosibirsk, Russian Federation.
Bread wheat (Triticum aestivum L.) is one of the most important agricultural plants wearing abiotic stresses, such as water deficit and cold, that cause its productivity reduction. Since resistance to abiotic factors is a multigenic trait, therefore modern genome-wide approaches can help to involve various genetic material in breeding. One technique is full transcriptome analysis that reveals groups of stress response genes serving marker-assisted selection markers. Comparing transcriptome profiles of the same genetic material under several stresses is essential and makes the whole picture. Here, we addressed this by studying the transcriptomic response to water deficit and cold stress for two evolutionarily distant bread wheat varieties: stress-resistant cv. Saratovskaya 29 (S29) and stress-sensitive cv. Yanetzkis Probat (YP). For the first time, transcriptomes for these cultivars grown under abiotic stress conditions were obtained using Illumina based MACE technology. We identified groups of genes involved in response to cold and water deficiency stresses, including responses to each stress factor and both factors simultaneously that may be candidates for resistance genes. We discovered a core group of genes that have a similar pattern of stress-induced expression changes. The particular expression pattern was revealed not only for the studied varieties but also for the published transcriptomic data on cv. Jing 411 and cv. Fielder. Comparative transcriptome profiling of cv. S29 and cv. YP in response to water deficit and cold stress confirmed the hypothesis that stress-induced expression change is unequal within a homeologous gene group. As a rule, at least one changed significantly while the others had a relatively lower expression. Also, we found several SNPs distributed throughout the genomes of cv. S29 and cv. YP and distinguished the studied varieties from each other and the reference cv. Chinese Spring. Our results provide new data for genomics-assisted breeding of stress-tolerant wheat cultivars.
PMID: 34026365
J Plant Res , IF:2.185 , 2021 May doi: 10.1007/s10265-021-01309-0
Characterization of a transcription factor SlNAC7 gene from Suaeda liaotungensis and its role in stress tolerance.
School of Life Sciences, Liaoning Normal University, Dalian, 116081, China.; Key Laboratory of Plant Biotechnology of Liaoning Province, Liaoning Normal University, Dalian, 116081, China.; School of Life Sciences, Liaoning Normal University, Dalian, 116081, China. skyliqiuli@163.com.; Key Laboratory of Plant Biotechnology of Liaoning Province, Liaoning Normal University, Dalian, 116081, China. skyliqiuli@163.com.
NAC (NAM, ATAF1/2, CUC2) transcription factors play important roles in plant growth, development, and responses to abiotic stress. In this study, we cloned an NAC2 subfamily transcription factor gene (SlNAC7) from the halophyte Suaeda liaotungensis K., and conducted a series of studies to determine the characteristics and functions of this gene. The SlNAC7 coding region contains 1719 base pairs that encode a 573 amino acid long protein. SlNAC7 is expressed in the roots, stems, and leaves of S. liaotungensis, with the highest expression in the leaves. We found that SlNAC7 expression can be induced by drought, salt, cold, and abscisic acid. Transient expression in onion epidermal cells revealed that SlNAC7 is located in both the nucleus and cytoplasm. A transcriptional activation experiment in yeast showed that the transcriptional activation domain of SlNAC7 is located at the C terminus. When SlNAC7 was transformed into Arabidopsis under the control of a CaMV 35S promoter its overexpression was found to enhance the ability of transgenic plants to resist drought, salt, and cold stress. Moreover, these plants showed multiple changes in growth characteristics and physiological and biochemical indices in response to different stresses, as well as the upregulation of numerous stress-related genes. We have thus characterized a new halophyte-derived NAC transcription factor, SlNAC7, which can regulate plant growth and physiological and biochemical changes under adverse conditions by regulating the expression of stress-related genes, thereby enhancing plant stress resistance. SlNAC7 is a promising candidate for breeding new varieties of stress-tolerant crops.
PMID: 33963939
Plant Signal Behav , IF:1.671 , 2021 May , V16 (5) : P1893978 doi: 10.1080/15592324.2021.1893978
HOS15-PWR chromatin remodeling complex positively regulates cold stress in Arabidopsis.
Institute of Glocal Disease Control, Konkuk University, Seoul, Republic of Korea.; Department of Biomedical Science and Engineering, Konkuk University, Seoul, Republic of Korea.; Division of Applied Life Science (Bk21plus), Plant Molecular Biology and Biotechnology Research Center, Research Institute of Life Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Cold stress is a major environmental constraint that restrains plant growth and productivity. To cope with cold stress, plants must be able to perceive a cold signal and regulate the expression of cold-regulated (COR) genes. In our recent study, we showed that Arabidopsis HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15) acts as a substrate receptor for CULLIN4-based ubiquitin E3 ligase complex to promote cold-induced histone deacetylase 2 C (HD2C) degradation that allows the activation of COR genes. Additionally, we found that POWERDRESS (PWR), a HOS15-interacting protein, is required for the association of HOS15 with COR gene chromatin and HD2C degradation. The HOS15/PWR complex interacts with and recruits CBF transcription factors to the promoters of COR genes. Collectively, our previous findings suggest that HOS15 and PWR function as positive regulators for the expression of COR genes, and promote cold tolerance. Accordingly, we herein discuss the role of PWR in cold tolerance.
PMID: 33641608