Plant Cell , IF:9.618 , 2020 Aug doi: 10.1105/tpc.20.00195
COR27 and COR28 are Novel Regulators of the COP1-HY5 Regulatory Hub and Photomorphogenesis in Arabidopsis.
Chinese Academy of Sciences CITY: Shanghai China [CN].; Institute of Plant Physiology and Ecology, SIBS CITY: Shanghai China [CN].; Chinese Academy of Sciences CITY: Shanghai.; Chinese Academy of Sciences CITY: Shanghai STATE: Shanghai China [CN].; Chinese Academy of Sciences CITY: Shanghai POSTAL_CODE: 200032 China [CN] htliu@cemps.ac.cn.
Plants have evolved sensitive signaling systems to fine-tune photomorphogenesis in response to changing light environments. Light and low temperatures are known to regulate the expression of the COLD REGULATED (COR) genes COR27 and COR28, which influence the circadian clock, freezing tolerance, and flowering time. Blue light stabilizes the COR27 and COR28 proteins, but the underlying mechanism is unknown. We therefore performed a yeast two-hybrid screen using COR27- and COR28 as bait, and identified the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) as an interactor. COR27 and COR28 physically interact with COP1, which is in turn responsible for their degradation in the dark. Furthermore, COR27 and COR28 promote hypocotyl elongation and act as negative regulators of photomorphogenesis in Arabidopsis. Genome-wide gene expression analysis showed that HY5, COR27, and COR28 co-regulate many common genes. COR27 interacts directly with HY5 and associate with the promoters of the HY5 target genes HY5 and PIF4, and regulates their transcription together with HY5. Our results demonstrate that COR27 and COR28 act as key regulators in the COP1-HY5 regulatory hub, by regulating the transcription of HY5 target genes together with HY5 to ensure proper skotomorphogenic growth in the dark and photomorphogenic development in the light.
PMID: 32769132
Plant Cell Environ , IF:6.362 , 2020 Aug doi: 10.1111/pce.13868
The HY5 and MYB15 transcription factors positively regulate cold tolerance in tomato via the CBF pathway.
Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou, China.; College of Forestry, Henan University of Science and Technology, 263 Kaiyuan Avenue, Luoyang, P.R.; Key Laboratory of Plant Growth, Development and Quality Improvement, Agricultural Ministry of China, Yuhangtang Road 866, Hangzhou, China.; Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Yuhangtang Road 866, Hangzhou, China.
The induction of C-repeat binding factors (CBFs) is crucial for plant survival at low temperatures. Therefore, understanding the mechanisms that regulate CBF transcription is vital for the future development of crops with increased cold tolerance. Here, we provide evidence for the existence of a LONG HYPOCOTYL 5 (HY5)-MYB15-CBFs transcriptional cascade that plays a crucial role in the cold response in tomato. The exposure of tomato plants to cold (4 degrees C) increased the levels of HY5, MYB15 and CBFs transcripts. Moreover, mutations in HY5 or MYB15 decreased the levels of CBF transcripts. In contrast, overexpression of HY5 or MYB15 increased CBF transcript abundance. Crucially, the HY5 transcription factor activated the expression of MYB15 by directly binding to the promoter region, while both HY5 and MYB15 activated the expression of CBF1, CBF2 and CBF3. Taken together, these data show that HY5 can directly regulate CBF transcript levels, and also influence CBF expression indirectly via MYB15. The coordinated action of HY5 and MYB15 allows precise regulation of CBF expression and subsequent cold tolerance. These findings provide an improved understanding of the molecular mechanisms affording transcriptional regulation of CBFs, which can be exploited in the future to enhance cold tolerance in crops.
PMID: 32799321
J Exp Bot , IF:5.908 , 2020 Aug , V71 (16) : P5074-5086 doi: 10.1093/jxb/eraa215
Molybdenum induces alterations in the glycerolipidome that confer drought tolerance in wheat.
Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China.; Microelement Research Center, Huazhong Agricultural University, Wuhan, China.; Department of Biology, University of Missouri, St. Louis, Missouri, USA.; Donald Danforth Plant Science Center, St. Louis, Missouri, USA.
Molybdenum (Mo), which is an essential microelement for plant growth, plays important roles in multiple metabolic and physiological processes, including responses to drought and cold stress in wheat. Lipids also have crucial roles in plant adaptions to abiotic stresses. The aim of this study was to use glycerolipidomic and transcriptomic analyses to determine the changes in lipids induced by Mo that are associated with Mo-enhanced drought tolerance in wheat. Mo treatments increased the transcript levels of genes involved in fatty acid and glycerolipid biosynthesis and desaturation, but suppressed the expression of genes involved in oxylipin production. Wheat plants supplemented with Mo displayed higher contents of monogalactosyldiacyglycerol (MGDG), digalactosyldoacylglycerol (DGDG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and phosphatidylcholine (PC) with increased levels of unsaturation. The levels of MGDG, DGDG, PG, and PC increased under PEG-simulated drought (PSD), and the magnitude of the responses varied in the presence and absence of Mo. Mo increased the accumulation of the most abundant glycerolipid species of C36:6, C34:4, and C34:3 by increasing the expression of genes related to desaturation under PSD, and this contributed to maintaining the fluidity of membranes. In addition, Mo attenuated the decreases in the ratios of DGDG/MGDG and PC/PE that were observed under PSD. These changes in lipids in Mo-treated wheat would contribute to maintaining the integrity of membranes and to protecting the photosynthetic apparatus, thus acting together to enhance drought tolerance.
PMID: 32369576
Int J Mol Sci , IF:4.556 , 2020 Aug , V21 (15) doi: 10.3390/ijms21155587
The Alleviation of Photosynthetic Damage in Tomato under Drought and Cold Stress by High CO2 and Melatonin.
Department of Food Science, Aarhus University, 8200 Aarhus N, Denmark.; Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.; College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.; Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.; Department of Plant and Environmental Sciences, University of Copenhagen, 2630 Taastrup, Denmark.
The atmospheric CO2 concentration (a[CO2]) is increasing at an unprecedented pace. Exogenous melatonin plays positive roles in the response of plants to abiotic stresses, including drought and cold. The effect of elevated CO2 concentration (e[CO2]) accompanied by exogenous melatonin on plants under drought and cold stresses remains unknown. Here, tomato plants were grown under a[CO2] and e[CO2], with half of the plants pre-treated with melatonin. The plants were subsequently treated with drought stress followed by cold stress. The results showed that a decreased net photosynthetic rate (PN) was aggravated by a prolonged water deficit. The PN was partially restored after recovery from drought but stayed low under a successive cold stress. Starch content was downregulated by drought but upregulated by cold. The e[CO2] enhanced PN of the plants under non-stressed conditions, and moderate drought and recovery but not severe drought. Stomatal conductance (gs) and the transpiration rate (E) was less inhibited by drought under e[CO2] than under a[CO2]. Tomato grown under e[CO2] had better leaf cooling than under a[CO2] when subjected to drought. Moreover, melatonin enhanced PN during recovery from drought and cold stress, and enhanced biomass accumulation in tomato under e[CO2]. The chlorophyll a content in plants treated with melatonin was higher than in non-treated plants under e[CO2] during cold stress. Our findings will improve the knowledge on plant responses to abiotic stresses in a future [CO2]-rich environment accompanied by exogenous melatonin.
PMID: 32759822
Microorganisms , IF:4.152 , 2020 Aug , V8 (8) doi: 10.3390/microorganisms8081213
Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics.
Laboratorio de Bioinformatica y Expresion Genica, Instituto de Nutricion y Tecnologia de los Alimentos, Universidad de Chile, El Libano 5524, 7810000 Santiago, Chile.; Center for Genome Regulation, El Libano 5524, Santiago 7810000, Chile.; Programa de Doctorado en Ciencias Silvoagropecuarias y Veterinarias, Campus Sur Universidad de Chile. Santa Rosa 11315, 8820808 Santiago, Chile.; GEMA Center for Genomics, Ecology and Environment, Universidad Mayor, Camino La Piramide 5750, 8320000 Santiago, Chile.; Laboratorio de Genomica y Genetica de Interacciones Biologicas (LG2IB). Instituto de Nutricion y Tecnologia de los Alimento, Universidad de Chile. El Libano 5524, 7810000 Santiago, Chile.
The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteria from two Chilean desert environments and characterize the beneficial traits for plants through a biochemical approach. By means of different culture strategies, we obtained 39 bacterial soil isolates from the Coppermine Peninsula (Antarctica) and 32 from Lejia Lake shore soil (Atacama Desert). The results obtained from the taxonomic classification and phylogenetic analysis based on 16S rDNA sequences indicated that the isolates belonged to four phyla (Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes), and that the most represented genus at both sites was Pseudomonas. Regarding biochemical characterization, all strains displayed in vitro PGP capabilities, but these were in different proportions that grouped them according to their site of origin. This study contributes with microbial isolates from natural extreme environments with biotechnological potentials in improving plant growth under cold stress.
PMID: 32785053
BMC Genomics , IF:3.594 , 2020 Aug , V21 (1) : P532 doi: 10.1186/s12864-020-06941-z
Comparative transcriptome analysis reveals ecological adaption of cold tolerance in northward invasion of Alternanthera philoxeroides.
School of Ecology and Environmental Science, Institute of Ecology and Geobotany, Yunnan University, Kunming, 650504, China.; Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.; Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.; University of Chinese Academy of Sciences, Beijing, 100049, China.; School of Ecology and Environmental Science, Institute of Ecology and Geobotany, Yunnan University, Kunming, 650504, China. yupenggeng@qq.com.; Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China. yangyp@mail.kib.ac.cn.; Plant Germplasm and Genomics Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China. yangyp@mail.kib.ac.cn.
BACKGROUND: Alternanthera philoxeroides (alligator weed) is a highly invasive alien plant that has continuously and successfully expanded from the tropical to the temperate regions of China via asexual reproduction. During this process, the continuous decrease in temperature has been a key limiting environmental factor. RESULTS: In this study, we provide a comprehensive analysis of the cold tolerance of alligator weed via transcriptomics. The transcriptomic differences between the southernmost population and the northernmost population of China were compared at different time points of cold treatments. GO enrichment and KEGG pathway analyses showed that the alligator weed transcriptional response to cold stress is associated with genes encoding protein kinases, transcription factors, plant-pathogen interactions, plant hormone signal transduction and metabolic processes. Although members of the same gene family were often expressed in both populations, the levels of gene expression between them varied. Further ChIP experiments indicated that histone epigenetic modification changes at the candidate transcription factor gene loci are accompanied by differences in gene expression in response to cold, without variation in the coding sequences of these genes in these two populations. These results suggest that histone changes may contribute to the cold-responsive gene expression divergence between these two populations to provide the most beneficial response to chilling stimuli. CONCLUSION: We demonstrated that the major alterations in gene expression levels belonging to the main cold-resistance response processes may be responsible for the divergence in the cold resistance of these two populations. During this process, histone modifications in cold-responsive genes have the potential to drive the major alterations in cold adaption necessary for the northward expansion of alligator weed.
PMID: 32741374
BMC Plant Biol , IF:3.497 , 2020 Aug , V20 (1) : P371 doi: 10.1186/s12870-020-02569-z
Transcriptomic profiling of germinating seeds under cold stress and characterization of the cold-tolerant gene LTG5 in rice.
Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, China.; Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, China.; Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, China. dengguofu163@163.com.
BACKGROUND: Low temperature is a limiting factor of rice productivity and geographical distribution. Wild rice (Oryza rufipogon Griff.) is an important germplasm resource for rice improvement. It has superior tolerance to many abiotic stresses, including cold stress, but little is known about the mechanism underlying its resistance to cold. RESULTS: This study elucidated the molecular genetic mechanisms of wild rice in tolerating low temperature. Comprehensive transcriptome profiles of two rice genotypes (cold-sensitive ce 253 and cold-tolerant Y12-4) at the germinating stage under cold stress were comparatively analyzed. A total of 42.44-68.71 million readings were obtained, resulting in the alignment of 29,128 and 30,131 genes in genotypes 253 and Y12-4, respectively. Many common and differentially expressed genes (DEGs) were analyzed in the cold-sensitive and cold-tolerant genotypes. Results showed more upregulated DEGs in the cold-tolerant genotype than in the cold-sensitive genotype at four stages under cold stress. Gene ontology enrichment analyses based on cellular process, metabolic process, response stimulus, membrane part, and catalytic activity indicated more upregulated genes than downregulated ones in the cold-tolerant genotype than in the cold-sensitive genotype. Quantitative real-time polymerase chain reaction was performed on seven randomly selected DEGs to confirm the RNA Sequencing (RNA-seq) data. These genes showed similar expression patterns corresponding with the RNA-Seq method. Weighted gene co-expression network analysis (WGCNA) revealed Y12-4 showed more positive genes than 253 under cold stress. We also explored the cold tolerance gene LTG5 (Low Temperature Growth 5) encoding a UDP-glucosyltransferase. The overexpression of the LTG5 gene conferred cold tolerance to indica rice. CONCLUSION: Gene resources related to cold stress from wild rice can be valuable for improving the cold tolerance of crops.
PMID: 32762649
Gene , IF:2.984 , 2020 Aug , V753 : P144797 doi: 10.1016/j.gene.2020.144797
Genomic and expression analysis indicate the involvement of phospholipase C family in abiotic stress signaling in chickpea (Cicer arietinum).
National Institute of Plant Genome Research, New Delhi 110067, India.; National Institute of Plant Genome Research, New Delhi 110067, India. Electronic address: amarjeet.singh@nipgr.ac.in.
Phospholipase C proteins are phospholipid hydrolysing enzymes and crucial components of abiotic stress triggered lipid signaling in plants. PLCs are implicated in plant reaction to drought, salinity, and cold stress responses, however, characterization of the PLC family in the legume crop chickpea is missing. Here, we identify and describe nine PLC encoding genes in the chickpea genome. Phylogenetic analysis showed that the chickpea PLC family has evolved through a common path in dicots. Subcellular localization of fluorescence tagged proteins confirmed cytoplasmic and plasma membrane bound forms of PLCs in chickpea. The promoters of all the PLC genes are comprised of several hormone response related, development and abiotic stress related cis-regulatory elements. Expression analysis in five developmental stages (germination, seedling, vegetative, reproductive and senescence) showed significant expression of multiple PLCs in germination, vegetative and reproductive stages, suggesting their diverse role in various developmental processes. qRT-PCR expression analysis of the entire PLC gene family under drought, salt and cold stresses revealed that most PLC genes are differentially expressed in multiple abiotic stresses. These observations indicate the involvement of PLC gene family in abiotic stress signaling and responses in important legume crop. The present study opens new avenues for utilizing PLC- related information in biotechnological programs for abiotic stress tolerance and legume crop improvement.
PMID: 32454180
Gene , IF:2.984 , 2020 Aug , V753 : P144803 doi: 10.1016/j.gene.2020.144803
Characterization of the soybean R2R3-MYB transcription factor GmMYB81 and its functional roles under abiotic stresses.
College of Plant Science, Jilin University, Changchun 130062, Jilin, China.; National Tobacco Gene Research Center, Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, Henan, China.; College of Plant Science, Jilin University, Changchun 130062, Jilin, China. Electronic address: xuyanli@jlu.edu.cn.
R2R3-type MYBs are a key group of regulatory factors that control diverse developmental processes and stress tolerance in plants. Soybean is a major legume crop with the richness of seed protein and edible vegetable oil, and 244 R2R3-type MYBs have been identified in soybean. However, the knowledge regarding their functional roles has been greatly limited as yet. In this study, a novel R2R3-type MYB (GmMYB81) was functionally characterized in soybean, and it is closely related to two abiotic stress-associated regulators (AtMYB44 and AtMYB77). GmMYB81 transcripts not only differentially accumulated in soybean tissues and during embryo development, but also were significantly enhanced by drought, salt and cold stress. Histochemical GUS assay in Arabidopsis indicated that GmMYB81 promoter showed high activity in seedlings, rosette leaves, inflorescences, silique wall, mature anthers, roots, and germinating seeds. Further investigation indicated that over-expression of GmMYB81 in Arabidopsis caused auxin-associated phenotypes, including small flower and silique, more branch, and weakened apical dominance. Moreover, over-expression of GmMYB81 significantly elevated the rates of seed germination and green seedling under salt and drought stress, indicating that GmMYB81 might confer plant tolerance to salt and drought stress during seed germination. Additionally, protein interaction analysis showed that GmMYB81 interacts with the abiotic stress regulator GmSGF14l. Further observation indicated that they displayed similar expression patterns under drought and salt stress, suggesting GmMYB81 and GmSGF14l might cooperatively affect stress tolerance. These findings will facilitate future investigations of the regulatory mechanisms of GmMYB81 in response to plant stress tolerance, especially seed germination under abiotic stresses.
PMID: 32446917
Plants (Basel) , IF:2.762 , 2020 Aug , V9 (9) doi: 10.3390/plants9091071
An Abiotic Stress Responsive U-Box E3 Ubiquitin Ligase Is Involved in OsGI-Mediating Diurnal Rhythm Regulating Mechanism.
Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee University, Yongin 17104, Korea.
The plant U-box (PUB) protein is the E3 ligase that plays roles in the degradation or post-translational modification of target proteins. In rice, 77 U-box proteins were identified and divided into eight classes according to the domain configuration. We performed a phylogenomic analysis by integrating microarray expression data under abiotic stress to the phylogenetic tree context. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) expression analyses identified that eight, twelve, and eight PUB family genes are associated with responses to drought, salinity, and cold stress, respectively. In total, 16 genes showed increased expression in response to three abiotic stresses. Among them, the expression of OsPUB2 in class II and OsPUB33, OsPUB39, and OsPUB41 in class III increased in all three abiotic stresses, indicating their involvement in multiple abiotic stress regulation. In addition, we identified the circadian rhythmic expression for three out of 16 genes responding to abiotic stress through meta-microarray expression data analysis. Among them, OsPUB4 is predicted to be involved in the rice GIGANTEA (OsGI)-mediating diurnal rhythm regulating mechanism. In the last, we constructed predicted protein-protein interaction networks associated with OsPUB4 and OsGI. Our analysis provides essential information to improve environmental stress tolerance mediated by the PUB family members in rice.
PMID: 32825403
Plant Signal Behav , IF:1.671 , 2020 Aug , V15 (8) : P1780403 doi: 10.1080/15592324.2020.1780403
ABA enhanced cold tolerance of wheat 'dn1' via increasing ROS scavenging system.
College of Life Science, Northeast Agricultural University , Harbin, Heilongjiang, China.
Abscisic acid (ABA) is an important plant hormone that plays significant roles in cold tolerance regulation. However, whether ABAimproves cold tolerance by increasing the activities of antioxidant enzymes in wheat remains unknown. In this study,the activities of antioxidant enzymes of the winter wheat variety 'dongnongdongmai 1' ('dn1')afterthe application of exogenous ABA under low temperature (0 degrees C, -10 degrees C, -20 degrees C, and -25 degrees C) were investigated. Results showed that cold stress significantly increased H2O2 and relative conductivity, whileABA significantly reduced this effect. ABA enhanced cold tolerance in both leaves and rhizomes at -10 degrees C and -20 degrees Cby increasing CAT, SOD, POD, APX, GR, DHAR, and MDHAR. However, this tolerance was weakenedat -25 degrees C with decreasing ASA, GSH, APX, DHAR, and MDHARthan at-10 degrees C and -20 degrees C.POD, GR, and DHARlevels peaked at -10 degrees C, while CAT, SOD, GSH, APX, and MDHAR content in rhizomes peaked at -20 degrees C. The rate of returning green was significantly increased after ABA treatment than in controls (93.5% vs 83.6 %). In 'dn1', rhizomes had a higher cold tolerance than leaves. Thereby, exogenous ABA could enhance cold tolerance byincreasing the activities of antioxidant enzymes.
PMID: 32619128
Mol Biol Rep , IF:1.402 , 2020 Aug doi: 10.1007/s11033-020-05714-x
Screening of optimal reference genes for qRT-PCR and preliminary exploration of cold resistance mechanisms in Prunus mume and Prunus sibirica varieties.
Beijing Advanced Innovation Center for Tree Breeding By Molecular Design, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, P.O. Box 155, Beijing, 100083, China.; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China.; National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China.; Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China.; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China.; School of Landscape Architecture, Beijing Forestry University, Beijing, China.; Beijing Advanced Innovation Center for Tree Breeding By Molecular Design, Beijing Forestry University, No. 35 Tsinghua East Road, Haidian District, P.O. Box 155, Beijing, 100083, China. zqxbjfu@126.com.; Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing, China. zqxbjfu@126.com.; National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, China. zqxbjfu@126.com.; Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China. zqxbjfu@126.com.; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing, China. zqxbjfu@126.com.; School of Landscape Architecture, Beijing Forestry University, Beijing, China. zqxbjfu@126.com.
Prunus sibirica and Prunus mume are closely related plant species that differ in cold tolerance. Hybrids of P. sibirica and true mume, belonging to the apricot mei group, inherited strong cold resistance from P. sibirica. These materials are favourable for research on the molecular mechanisms of cold resistance. However, no suitable reference genes have been identified for analysing gene expression patterns between P. sibirica and P. mume. Ten candidate reference genes were assessed, namely, actins (ACT2-1, ACT2-2, ACT2-3, ACT2-4), protein phosphatase 2A-1 (PP2A-1), ubiquitins (UBQ2, UBQ3), ubiquitin extension protein (UBQ1) and tubulins (TUB1, TUB2), with four distinct algorithms (geNorm, NormFinder, BestKeeper and RefFinder). UBQ2 was recognized as the best reference gene in stems and buds across materials (P. sibirica; 'Xiaohong Zhusha', 'Beijing Yudie', and 'Xiao Lve' for true mume; and 'Dan Fenghou', 'Fenghou', and 'Yanxing' for apricot mei) under cold stress. In addition, the temporal and spatial expression patterns of PmCBF6 and PmLEA10 among seven varieties during winter periods were analysed using UBQ2 as a reference gene. The expression differed significantly among cultivars, which may contribute to their differences in cold tolerance. This paper confirmed the strong cold tolerance of apricot mei. And the best internal reference gene suitable for seven varieties was selected: UBQ2. Based on the above results, the expression of PmCBF6 and PmLEA10 genes during wintering in seven varieties was analysed. The molecular mechanisms of cold resistance were found to be possibly different in different varieties of P. sibirica and P. mume.
PMID: 32803506